EFFICIENCY ESTIMATION OF “BIO-GIDE” RESORBABLE MEMBRANE APPLICATION WITH CHITOSAN OF VARIOUS THICVKNESS IN COMBINATION WITH “BIO-OSS” OSTEOPLASTIC MATERIAL FOR BONE TISSUE DEFECTS TREATMENT 

Kalmin Oleg Vital'evich, Doctor of medical sciences, professor, head of sub-department of human anatomy, Medical Institute, Penza State University (40 Krasnaya street, Penza, Russia), ovkalmin@gmail.com
Nikishin Dmitriy Viktorovich, Candidate of medical sciences, associate professor, sub-department of human anatomy, Medical Institute, Penza State University (40 Krasnaya street, Penza, Russia), nikishindv@gmail.com
Volodina Yuliya Mikhaylovna, Assistant, sub-department of human anatomy, Medical Institute, Penza State University (40 Krasnaya street, Penza, Russia), yafonasova@mail.ru

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Background. The article aims at evaluating the efficiency of a «Bio-Gide» resorbable membrane application with chitosan of different thickness combined with a «Bio-Oss» osteoplastic material for regeneration of destructed bone tissues in experimental conditions.
Materials and methods. The authors histologically examined tissue samples from 9 mature rabbits from the area of artificial defects of mandible after 14, 28 and 42 days since the surgery. In group 1, treatment was carried out using the membrane with 200×3 (thin) chitosan, in 2nd group the researchers applied the membrane with 200×3 (thin) chitosan in combination with the «Bio-Oss» osteoplastic materials, in the third group the membrane with 200×38 (thick) chitosan was used, in the 4th – the membrane with 200×38 (thick) chitosan combined with the «Bio-Oss» osteoplastic material.
Results. The study revealed that in the usage of chitosan membranes the efficiency of osteoregeneration of the above mentioned samples is higher with the membranes of greater thickness. The percentage of bone tissue at the end of the experiment was higher in the samples with the «Bio-Oss» osteoplastic material application; in the same samples there occurred vascularization.
Conclusion. Usage of xenopericardial membranes with chitosan combined with osteoplastic material «Bio-Oss» has a positive impact on the recovery process of the bone tissue defects. The neoplasm of bone tissues in all samples takes place avoiding the stage of cartilage development. By the end of the experiment the chitosan membranes completely degraded and were not detected in the samples.

xenopericardium, bone tissue, connective tissue, regeneration, bone tissue defect, chitosan.

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